GE27-5330-02
Microspin™ G-50 Columns
Cytiva 27-5330-02, pack of 250 columns
Synonym(s):
Microspin columns
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
packaging
pack of 250 columns
manufacturer/tradename
Cytiva 27-5330-02
particle size
50-150 μm (dry)
Related Categories
General description
MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.
Features and Benefits
- For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography.
- Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
- Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
- Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
Regulatory Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Domagoj Baretić et al.
Molecular cell, 78(5), 926-940 (2020-05-06)
The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase, orchestrates chromosome replication. Multiple factors associate directly with CMG, including Ctf4 and the heterotrimeric fork protection complex (Csm3/Tof1 and Mrc1), which has important roles including aiding normal replication rates and stabilizing
Protocols
DNA cleanup using illustra™ GFX kits from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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