GE25-6400-10
TempliPhi™ 100 Amplification Kit
Cytiva 25-6400-10
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UNSPSC Code:
41116006
NACRES:
NA.85
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
manufacturer/tradename
Cytiva 25-6400-10
storage condition
(The product can be s-red for 2 months or less at -20°C. For s-rage longer than 2 months, s-re at -70°C Thaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior - amplification)
shipped in
dry ice
storage temp.
−70°C
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General description
illustra™ TempliPhi™ DNA Amplification Kits use isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates.
TempliPhi™ Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30° C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.
Two different types of TempliPhi™ DNA Amplification Kits are available to meet different throughput requirements. illustra™ TempliPhi™ DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability.
The illustra™ TempliPhi™ DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed.
TempliPhi™ Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30° C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.
Two different types of TempliPhi™ DNA Amplification Kits are available to meet different throughput requirements. illustra™ TempliPhi™ DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability.
The illustra™ TempliPhi™ DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed.
Application
Preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
Features and Benefits
- Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h.
- Generate microgram quantities of template DNA from picogram amounts of starting material.
- Use amplified DNA directly for cycle sequencing without purification.
- Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA.
- Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation.
- For low-to medium-throughput, small DNA vector preparation.
Storage and Stability
The product can be s-red for 2 months or less at -20°C. For s-rage longer than 2 months, s-re at -70°C Thaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior - amplification
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
TempliPhi
On packaging and insert for kits:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and their equivalents in other countries.
On packaging and insert for kits:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and their equivalents in other countries.
TempliPhi is a trademark of Cytiva
illustra is a trademark of Cytiva
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Abraham J Kandathil et al.
Nature communications, 12(1), 6909-6909 (2021-11-27)
There is an urgent need for innovative methods to reduce transmission of bloodborne pathogens like HIV and HCV among people who inject drugs (PWID). We investigate if PWID who acquire non-pathogenic bloodborne viruses like anelloviruses and pegiviruses might be at
Sha Tang et al.
Journal of nematology, 37(2), 236-241 (2005-06-01)
To enable investigation of nematode mitochondrial DNA evolution, methodology has been developed to amplify intact nematode mitochondrial genomes in preparative yields using a rolling circle replication strategy. Successful reactions were generated from whole cell template DNA prepared by alkaline lysis
Michael J Tisza et al.
eLife, 9 (2020-02-06)
Although millions of distinct virus species likely exist, only approximately 9000 are catalogued in GenBank's RefSeq database. We selectively enriched for the genomes of circular DNA viruses in over 70 animal samples, ranging from nematodes to human tissue specimens. A
Tracy L Meiring et al.
Virology journal, 9, 164-164 (2012-08-18)
Human papillomavirus (HPV) is the aetiological agent for cervical cancer and genital warts. Concurrent HPV and HIV infection in the South African population is high. HIV positive (+) women are often infected with multiple, rare and undetermined HPV types. Data
Host-Pathogen Interactions in Human Polyomavirus 7‒Associated Pruritic Skin Eruption.
Rachel K Rosenstein et al.
The Journal of investigative dermatology, 141(5), 1344-1348 (2020-10-20)
Protocols
This page shows circular DNA amplification using TempliPhi™ from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service