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GE17-6003-18

DeStreak Reagent

Cytiva 17-6003-18

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EC Number:
UNSPSC Code:
41105323
NACRES:
NA.25

form

liquid

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

wetted part: no

packaging

pkg of 1 mL

manufacturer/tradename

Cytiva 17-6003-18

storage condition

(Rehydration Solution -20)

color

Colorless

storage temp.

2-8°C

Related Categories

General description

DeStreak Reagent.

Application

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Features and Benefits

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

DeStreak is a trademark of Cytiva

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Eye Dam. 1

WGK

WGK 3

Flash Point(F)

233.6 °F

Flash Point(C)

112 °C

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocols

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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