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Merck
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GE17-5478-55

HiTrap® Protein L

Cytiva 17-5478-55, pack of 5 × 5 mL

Synonym(s):

HiTrap Protein L, Cytiva chromatography column, FPLC column, affinity column, antibody fragment purification, kappa light chain

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816

Key Documents

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Technical Service
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ligand

recombinant protein L (E. coli), mammalian free

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5478-55

parameter

5 bar

column I.D.

16 mm

matrix

rigid, highly cross-linked agarose

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

working range

2-10(pH interval where the medium can be operated without significant change in function.)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

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General description

HiTrap® Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.

Features and Benefits

  • Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).
  • Enables a platform approach to antibody fragment purification.
  • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
  • Convenient for process development, as well as small-scale purification.
  • HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Performing a Purification of Antibody Fragments with Capto™ L Products

This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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