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GE17-5478-51

HiTrap® Protein L

Cytiva 17-5478-51, pack of 5 × 1 mL

Synonym(s):

HiTrap Chromatography Column, Protein L Column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

recombinant protein L (E. coli), mammalian free

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5478-51

storage condition

(20% Ehtanol)

parameter

5 bar

column I.D.

7 mm

matrix

rigid, highly cross-linked agarose

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

working range

2-10(pH interval where the medium can be operated without significant change in function.)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

Related Categories

General description

HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
HiTrap® Protein L columns are packed with Capto™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.

Application

HiTrap® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).

Features and Benefits

  • Captures a wide range of antibody fragments
  • Allows a platform approach for the purification of antibody fragment
  • Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
  • Reduces process time and amount of resin
  • Suitable for process development and small-scale purification
  • HiTrap® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA® system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Purification of Antibody Fragments with Capto™ L Products

This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service