GE17-5280-01
nProtein A Sepharose™ 4 Fast Flow
Cytiva 17-5280-01, pack of 5 mL
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ligand
native protein A (S. aureus)
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-5280-01
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
average diameter
90 μm (d50v)
cleaning
2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9
capacity
>30 mg binding capacity(human IgG/ml)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
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General description
nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.
nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
- Used in routine commercial production of monoclonal antibodies
- Free from animal-derived components.
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Certificates of Analysis (COA)
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Articles
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Protocols
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Related Content
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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