GE17-5279-01
Glutathione Sepharose™ High Performance
Cytiva 17-5279-01, pack of 25 mL
Recommended Products
ligand
glutathione
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5279-01
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity(medium)
Related Categories
General description
Glutathione Sepharose™ High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.
Features and Benefits
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
- Mild conditions during the purification process preserves protein antigenicity and function
Storage and Stability
Analysis Note
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Regulatory Information
Certificates of Analysis (COA)
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Articles
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protocols
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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