GE17-5255-01
HisTrap™ Fast Flow
Cytiva 17-5255-01, pack of 5 × 5 mL
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5255-01
parameter
<4 mL/min flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
Related Categories
General description
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Features and Benefits
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Storage and Stability
Analysis Note
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Articles
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
This page shows how to purify protein A-tagged proteins using Cytiva products.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
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