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GE17-5175-01

Superdex® 200 10/300 GL

Cytiva 17-5175-01, column L × I.D. 30 cm × 10 mm, 13 μm avg. part. size

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About This Item

UNSPSC Code:
12352200

material

borosilicate glass column
polypropylene filter

mol wt

10000-600000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-5175-01

parameter

0.25-0.75 mL/min flow rate
1.5 Mpa (217 psi)
25-500 mL sample volume

technique(s)

HPLC: suitable

bed volume

24 mL

column L × I.D.

30 cm × 10 mm

matrix

agarose-dextran composite

avg. part. size

13 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex® 200 10/300 GL is a prepacked gel filtration column for high-resolution, semipreperative and analytical separations of biomolecules. Superdex® 200 has a separation range for molecules with molecular weights between 10 000 and 600 000.

Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.

Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.

Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family, that provideexcellent reproducibility and durability in purification using a chromatography system.

Features and Benefits

  • High-resolution separations of proteins, peptides, and other biomolecules according to size.
  • Highly suitable for the polishing step in a purification procedure.
  • Easy and predictable scale-up.
  • Excellent reproducibility and durability.
  • For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

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Regulatory Information

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