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GE17-5174-01

Sigma-Aldrich

Superdex® 75 10/300 GL

Cytiva 17-5174-01, column L × I.D. 30 cm × 10 mm, 13 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
polypropylene fiber (reinforced glass)

product line

Superdex®

mol wt

3000-70000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-5174-01

parameter

0.5 ml/min - 1 ml/min flow rate
1.8 Mpa (261 psi)
25-500 μL sample volume

bed volume

24 mL

column L × I.D.

30 cm × 10 mm

matrix

agarose-dextran composite
polymeric

avg. part. size

13 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex® 75 10/300 GL is a prepacked gel filtration column for high-resolution, semipreparative, and analytical separations of biomolecules. Superdex® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000.

Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.

Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.

Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family excellent reproducibility and durability in purification using a chromatography system.

Features and Benefits

  • High-resolution separations of proteins, peptides, and other biomolecules according to size.
  • Well suited for the polishing step in a purification procedure.
  • Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
  • Easy and predictable scale-up.
  • Excellent reproducibility and durability.
  • For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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