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GE17-5173-01

Sigma-Aldrich

Superose® 12 10/300 GL

Cytiva 17-5173-01, column L × I.D. 30 cm × 10 mm, 11 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

borosilicate glass column
polypropylene filter

mol wt

1000-300000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-5173-01

parameter

0.5 ml/min - 1 ml/min flow rate
25-500 mL sample volume
3 Mpa (435 psi)

technique(s)

HPLC: suitable

bed volume

24 mL

column L × I.D.

30 cm × 10 mm

matrix

highly cross-linked agarose

matrix active group

agarose phase

avg. part. size

11 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superose® 12 10/300 GL are prepacked Tricorn columns for-high performance gel filtration with a broad fractionation range of molecules with molecular weights between 1000 and 300 000.

Features and Benefits

  • Achieve high-resolution separations across very broad molecular-weight ranges.
  • Low ionic strength eluents (<0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose® for some lipids, peptides, and small aromatic compounds.
  • Prepacked Tricorn columns are durable, high-performance glass columns for reliable and reproducible separations.
  • Easy, efficient scale-up from prepacked Superose® 10/300 GL columns to Superose® prep grade lab packs.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
for high-resolution separations across exceptionally broad molecular-weight ranges

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superose is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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