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GE17-5166-01

Sigma-Aldrich

Mono Q® 5/50 GL

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UNSPSC Code:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

material

PEEK column

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 1 ea

parameter

580 psi

bed volume

1 mL

column I.D.

5.7 mm

matrix

monodisperse porous polystyrene/divinyl benzene beads
polymeric

matrix active group

ammonium ion, quaternary phase

avg. part. size

10 μm

cleaning

1-14

working range

2-12

separation technique

anion exchange

General description

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Features and Benefits

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

See All

Protocols

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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