GE17-5162-01
HiTrap® Anx Fast Flow (high sub)
Cytiva 17-5162-01, pack of 5 × 1 mL
Recommended Products
ligand
diethylaminopropyl
description
Ion Exchanger Type (value)
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5162-01
parameter
<4 mL/min flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium
Related Categories
General description
Application
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
Features and Benefits
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High fLow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Storage and Stability
Analysis Note
Other Notes
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Warning
Hazard Statements
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Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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