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GE17-5138-01

rmp Protein A Sepharose™ Fast Flow

Cytiva 17-5138-01, pack of 5 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5138-01

storage condition

(20% Ehtanol)

matrix

4% cross-linked agarose

particle size

60-165 μm

average diameter

90 μm (d50v)

cleaning in place

2-11

working range

3-10

capacity

>35 mg binding capacity(human IgG/ml)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

rmp protein A Sepharose™ Fast FLow is a low leakage, non-mammalian based affinity medium designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.

Application

rmp Sepharose™ 4 Sepharose™ Fast FLow is composed of recombinant Protein A coupled to the well established Sepharose™ 4 Fast FLow base matrix by reductive amination resulting in multipoint attachment.

Reductive amination creates chemically stable amide bonds and resulting Low ligand leakage. rmp Protein A Sepharose™ 4 Fast FLow is manufactured without using animal-derived components. As member of the BioProcess media range, rmp Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Multi-point attachment of rProtein A by chemically-stable amide bonds
  • Coupling chemistry that minimizes leakage of Protein A ligand.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Robert Becker et al.
eLife, 10 (2021-10-05)
Non-centrosomal microtubule-organizing centers (MTOCs) are pivotal for the function of multiple cell types, but the processes initiating their formation are unknown. Here, we find that the transcription factor myogenin is required in murine myoblasts for the localization of MTOC proteins

Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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