GE17-5110-01
HiTrap® IGM purification
Cytiva 17-5110-01, pack of 5 × 1 mL
Synonym(s):
IGM Purification Column
About This Item
ligand
2-mercaptopyridine
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5110-01
storage condition
(20% Ethanol)
parameter
<4 mL/min flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
3-11
storage temp.
2-8°C
Related Categories
General description
Application
Features and Benefits
- Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
- Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
- Recovery of IgM is high with excellent activity retention.
- The top and bottom frits are manufactured from porous polyethylene
- Columns are delivered with a stopper on the inlet and a snap-off end on the outle
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
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