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Key Documents

Safety Information

GE17-5054-01

HiTrap® SP Fast Flow

Cytiva 17-5054-01, pack of 5 × 1 mL

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25 REACTIONS
CN¥2,002.48
100 REACTIONS
CN¥7,593.37
500 REACTIONS
CN¥37,946.95

CN¥2,002.48


Please contact Customer Service for Availability
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25 REACTIONS
CN¥2,002.48
100 REACTIONS
CN¥7,593.37
500 REACTIONS
CN¥37,946.95

About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

CN¥2,002.48


Please contact Customer Service for Availability
New, lower price on this item!

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5054-01

parameter

flow rate
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

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1 of 4

This Item
P4600MIRRT11483188001
usage

sufficient for 100 reactions

usage

sufficient for 100 reactions

usage

-

usage

sufficient for 30 reactions (including 5 control reactions)

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

-

feature

dNTPs included, hotstart: no

color

colorless

color

colorless

color

-

color

-

input

purified RNA

input

purified DNA

input

-

input

purified RNA

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

General description

HiTrap® SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

Features and Benefits

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

See All

Protocols

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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