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GE17-1196-01

Sigma-Aldrich

HiPrep™ 26/60 Sephacryl® S-300 HR

Cytiva 17-1196-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

Synonym(s):

HiPrep Chromatography Column, Sephacryl S-300 HR

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene (PP)

mol wt

10000-1500000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1196-01

parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa (22 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

General description

HiPrep™ 26/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
HiPrep 26/60 Sephacryl S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-300 is used for separating midsize proteins including antibodies and serum proteins.
for using preparative separation of biomolecules of up to 13 mL sample volume

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Regulatory Information

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Certificates of Analysis (COA)

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Protocols

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Sephacryl® Separation

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Related Content

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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