GE17-0980-01
Butyl Sepharose™ 4 Fast Flow
Cytiva 17-0980-01, pack of 200 mL
Synonym(s):
Butyl Sepharose, Fast Flow Sepharose
About This Item
ligand
butyl
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-0980-01
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
Related Categories
General description
Application
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Optimized for the separation of larger Proteins in capture and intermediate purification steps
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
This page discusses column packing and preparation techniques for reverse phase chromatography.
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
Protocols
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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