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Safety Information

GE17-0700-01

2′,5′ ADP-Sepharose™ 4B

Cytiva 17-0700-01, pack of 5 g

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 5 g

manufacturer/tradename

Cytiva 17-0700-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-10

working range

4-10

shipped in

wet ice

storage temp.

2-8°C

General description

2′5′ ADP Sepharose™ 4B media with immobilized NADP structural analog is primarily used for purification of enzymes requiring NADP as a cofactor.

Application

2′5′ ADP Sepharose™ 4B interacts strongly with NADP+-dependent dehydrogenases. Selective elution with gradients of NAD+ or NADP+ has allowed the resolution of complex mixtures of dehydrogenase isoenzymes using 2′5′ ADP Sepharose™ 4B.

Features and Benefits

  • 2′5′ ADP is an NADP structural analog immobilized on Sepharose™ 4B. It binds and immobilizes enzymes requiring this cofactor

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Regulatory Information

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Certificates of Analysis (COA)

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Articles

Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B

This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.

Converting from Flow Velocity to Volumetric Flow Rates

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Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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