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GE17-0640-01

Act Thiol Sepharose™ 4B

Cytiva 17-0640-01, pack of 15 g

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 15 g

manufacturer/tradename

Cytiva 17-0640-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-8

working range

2-8

storage temp.

2-8°C

General description

Activated Thiol Sepharose™ 4B medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules.

Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.

Features and Benefits

  • Reversible coupling of proteins and large biomolecules with thiol groups to Sepharose™ 4B via a glutathione spacer arm
  • The ligand is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBractivated Sepharose™ 4B.
  • Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids
  • Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
  • Separate thiol-containing proteins from non-thiol-containing proteins

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Regulatory Information

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Protocols

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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