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Product Name
Protein G Sepharose™ 4 Fast Flow, Cytiva 17-0618-02, pack of 25 mL
form
resin
crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0618-02
storage condition
(20% Ehtanol)
parameter
<0.1 Mpa pressure
150-250 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
4% cross-linked agarose
matrix active group
Recombinant streptococcal protein G lacking the albumin-binding region, produced in E. coli
average diameter
90 μm
cleaning in place
2-10
pH
3—9
working range
3-9
capacity
≥20 mg binding capacity(human IgG/mL resin)
separation technique
affinity
storage temp.
2-8°C
Related Categories
Analysis Note
Features and Benefits
- Binding specificities that complement Protein A media.
- Binds a broad range of IgG species and subclasses.
- Multi-point attachment minimizes ligand leakage.
- Used in a range of research applications.
General description
Protein G Sepharose™ 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose™ 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose™ 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.
As member of the BioProcess media range, Protein G Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
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This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Protocols
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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