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Safety Information

GE17-0506-01

Sigma-Aldrich

Mono Q® HR 16/10

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

material

PEEK column

description

Ion Exchanger Type (value)

packaging

pkg of 1 ea

parameter

435 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

monodisperse porous polystyrene/divinyl benzene beads
polymeric

matrix active group

ammonium ion, quaternary phase

avg. part. size

10 μm

cleaning

1-14

working range

2-12

separation technique

anion exchange

General description

Mono Q® HR 16/10 is a strong anion exchanger prepacked with MonoBeads. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required. The 20 mL bed volume of the column offers high capacity.

Features and Benefits

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

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Articles

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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