Product Name
Sephadex® G-50, Medium, Cytiva, pack of 500 g
SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
form
resin
crosslinking
cross-linked
mol wt
protein Mr 3-20 kDa (fractionation range)
protein Mr 30 kDa (exclusion limit)
Mp 0.5-10 kDa (dextrans fractionation range)
feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 500 g
manufacturer/tradename
Cytiva
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
matrix active group
dextran
swelling
1 g swells to 14-15 mL
particle size
103-320 μm (wet)
50-150 μm (dry)
cleaning in place
2-13
working range
2-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
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Analysis Note
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
- Classic gel filtration medium.
General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Preparation Note
Legal Information
Storage Class
13 - Non Combustible Solids
Regulatory Information
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Protocols
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。
Related Content
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
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