GE17-0042-01
Sephadex® G-50
Fine, Cytiva, pack of 100 g
About This Item
feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 100 g
manufacturer/tradename
Cytiva
parameter
flow rate
matrix
cross-linked dextran
swelling
1 g swells to 9-11 mL
particle size
20-80 μm (dry)
(wet)
cleaning in place
2-13
working range
2-10
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Small bead size for shorter diffusion distances
- Classic gel filtration medium.
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Regulatory Information
Certificates of Analysis (COA)
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