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Safety Information

GE11-0032-73

HiTrap® Capto™ MMC

Cytiva 11-0032-73, pack of 5 × 1 mL

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UNSPSC Code:
23151817
NACRES:
NA.56

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 11-0032-73

parameter

flow rate
<4 ml/min flow rate
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked agarose

avg. part. size

75 μm (d50v)

cleaning

2-14

working range

2-12

suitability

suitable for bioprocess medium

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General description

HiTrap® Capto™ MMC is prepacked with Capto™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Application

The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of Proteins regardless of ionic strength of the loading material . This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap® Capto™ MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.


The Capto™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.

Features and Benefits

  • High dynamic binding capacity at high conductivity.
  • Selectivity allows binding in the presence of salt.
  • High volume throughput.
  • HiTrap® Capto™ MMC can be used to bind Proteins at the conductivity of the feed material and to solve specific purification challenges.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Regulatory Information

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Certificates of Analysis (COA)

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Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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