Skip to Content
Merck
CN

G9880

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

Synonym(s):

ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
9000-86-6
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-561-4
MDL number:
MFCD28970979
Beilstein/REAXYS Number:
3562140
EC Number:
2.6.1.2 (BRENDA, IUBMB)

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Glutamic-Pyruvic Transaminase from porcine heart, ammonium sulfate suspension, ≥75 units/mg protein

biological source

Porcine heart

form

ammonium sulfate suspension

specific activity

≥75 units/mg protein

mol wt

42 kDa

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Physical form

Suspension in 1.8 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret.

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

Biochem/physiol Actions

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

General description

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

Other Notes

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000272438
0000257088
0000272224
0000272438
0000272224

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Glutamic-Pyruvic Transaminase 1 Facilitates Alternative Fuels for Hepatocellular Carcinoma Growth?A Small Molecule Inhibitor, Berberine
Guo W, et al.
Cancers, 12(7), 1854-1854 (2020)
Paradoxical Inhibition of Glycolysis by Pioglitazone Opposes the Mitochondriopathy Caused by AIF Deficiency
Benit P, et al.
EBioMedicine, 17, 75?87-75?87 (2017)
Detection of L-Lactate In Polyethylene Glycol Solutions Confirms Identity of Active Site Ligand in Proline Dehydrogenase Structure
Zhang M and Tanner JJ
Acta Crystallographica Section D, Biological Crystallography, 60(0 5), 985?986-985?986 (2004)
Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States
Zechel J, et al.
Journal of Separation Science, 79?98-79?98 null
Martin J J Ronis et al.
Experimental biology and medicine (Maywood, N.J.), 238(2), 151-162 (2013-04-12)
Metabolic syndrome is often accompanied by development of hepatic steatosis and less frequently by non-alcoholic fatty liver disease (NAFLD) leading to non-alcoholic steatohepatitis (NASH). Replacement of corn oil with medium chain triacylglycerols (MCT) in the diets of alcohol-fed rats has
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service