G9785
GSEM Supplement
liquid, suitable for cell culture,
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Synonym(s):
Glutamine synthetase expression medium supplement
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
concentration
50 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
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General description
Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium. It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.
Application
GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells and selective medium for NS0 myeloma cells.
Packaging
Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0
Quantity
One mL will supplement 50 mL of cell culture medium.
related product
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Soluble Fc fusion proteins for biomedical research
Monoclonal Antibodies: Methods and Protocols, 33-52 (2007)
Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies
Noh SM, et al.
Scientific reports, 8(1), 5361-5361 (2018)
A streamlined implementation of the glutamine synthetase-based protein expression system
Knox R, et al.
BMC biotechnology, 13(1), 74-74 (2013)
Novel membrane?bound reporter molecule for sorting high producer cells by flow cytometry.
Helman D, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 85(2), 162-168 (2014)
Doyle, A., et al.
Cell and Tissue Culture: Laboratory Procedures, 27D, 3-3 (1999)
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