Skip to Content

尊敬的客户：

目前国际形势复杂多变，关税政策尚不明朗，这可能对我们的产品价格产生一定影响。在此情况下，我们希望就订单事宜与您进行友好沟通。

基于当前的不确定性，如果您选择在此期间下单，我们将保留根据实际情况调整价格的权利。同时，我们也理解市场变化可能给您带来的困扰，因此如果在订单实际发货前因关税政策变动导致价格出现较大波动，默克将与您进行协商讨论并视情况对订单进行调整或取消。

关于应对近期政策变化的重要更新，请点击此处查看详情。

Merck
CN
All Photos(4)

Key Documents

View All Documentation

Safety Information

G9295

Sigma-Aldrich

Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse

clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate Dehydrogenase

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

MDL number:
MFCD09265360
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAPDH-71.1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken

should not react with

prokaryotes

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
726226922274549
乳酸标准液，用于离子色谱分析 TraceCERT®, 1000 mg/L lactate in water (nominal concentration)

07096

乳酸标准液，用于离子色谱分析

乳糖标准液，用于离子色谱分析 TraceCERT®, 1000 mg/L in water (nominal concentration)

72622

乳糖标准液，用于离子色谱分析

葡萄糖标准液，用于离子色谱分析 TraceCERT®, 1000 mg/L in water (nominal concentration)

69222

葡萄糖标准液，用于离子色谱分析

葡萄糖酸标准液，用于离子色谱分析 TraceCERT®, 1000 mg/L gluconate in water (nominal concentration)

74549

葡萄糖酸标准液，用于离子色谱分析

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

application(s)

environmental
food and beverages

application(s)

environmental
food and beverages

application(s)

environmental
food and beverages

format

single component solution

format

single component solution

format

single component solution

format

single component solution

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

concentration

1000 mg/L lactate in water (nominal concentration)

concentration

1000 mg/L in water (nominal concentration)

concentration

1000 mg/L in water (nominal concentration)

concentration

1000 mg/L gluconate in water (nominal concentration)

General description

Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.
The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.

Specificity

The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.

Immunogen

rabbit GAPDH.

Application

Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in
  • immunoblotting[1]
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000397435
    0000260424
    0000222464
    0000397435
    0000260424

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Jennifer L Whiting et al.
    Proceedings of the National Academy of Sciences of the United States of America, 113(30), E4328-E4337 (2016-07-13)
    Filtration through the kidney eliminates toxins, manages electrolyte balance, and controls water homeostasis. Reabsorption of water from the luminal fluid of the nephron occurs through aquaporin-2 (AQP2) water pores in principal cells that line the kidney-collecting duct. This vital process
    Bothrops jararaca venom metalloproteinases are essential for coagulopathy and increase plasma tissue factor levels during envenomation
    Yamashita KM, et al.
    PLoS Neglected Tropical Diseases, 8(5), e2814-e2814 (2014)
    Heterogeneous cadherin expression and multicellular aggregate dynamics in ovarian cancer dissemination
    Klymenko Y, et al.
    Neoplasia, 19(7), 549-563 (2017)
    Muscle Lim Protein and myosin binding protein C form a complex regulating muscle differentiation
    Arvanitis DA, et al.
    Biochimica et Biophysica Acta - Molecular Cell Research, 1864(12), 2308-2321 (2017)
    Naoya Yuhki et al.
    Genome research, 13(6A), 1169-1179 (2003-05-14)
    To study comparative molecular dynamics in the genesis of the major histocompatibility complex (MHC), we determined a complete nucleotide sequence spanning 758,291 bp of the domestic cat (Felis catus) extended and classical class II region. The feline class II MHC
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service