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Quality Level
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 12-15 mL gel
bead diameter
40-120 μm
application(s)
life science and biopharma
compatibility
Cytiva
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General description
Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.
Application
Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.
Features and Benefits
- Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
- Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
- Features the highest exclusion limit of 80,000 within the Sephadex G-range.
- Classic gel filtration resin.
Legal Information
Sephadex is a registered trademark of Cytiva
replaced by
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
K Krakowski et al.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
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Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
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Journal of biological and chemical chronicles, 266(34), 23323-23328 (1991)
Jose Munoz-Munoz et al.
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Effects of LDL on intracellular free calcium and nitric oxide-dependent cGMP formation in porcine endothelial cells
Pohl U, et al.
Atherosclerosis, 117(2), 169-178 (1995)
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