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Safety Information

G7509

Sigma-Aldrich

GABase from Pseudomonas fluorescens

lyophilized powder, Protein ≥30 % by biuret

Synonym(s):

GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase

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About This Item

MDL number:
MFCD00131113
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

Pseudomonas fluorescens

Quality Level

200

Assay

≥30  % (biuret)

form

lyophilized powder

specific activity

≥0.5 units/mg protein

composition

Protein, ≥30% biuret

technique(s)

inhibition assay: suitable

storage temp.

−20°C

General description

Research area: Neuroscience


GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.

Application

GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).

Biochem/physiol Actions

GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.

Unit Definition

One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000375983
0000359433
0000340581
0000340581
0000359433

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?

    Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.

  6. What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?

    Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.

  7. Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?

    The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Glutamate (GLU) and gamma-aminobutyric acid (GABA) are neurotransmitters (NTs) with an essential role in signal transmission in the brain. Brain disorders, such as epilepsy, Alzheimer's and Parkinson's diseases, and traumatic brain injury can be linked to imbalances in the GLU-GABA
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