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Merck
CN

G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym(s):

sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

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About This Item

CAS Number:
9075-65-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-982-3
MDL number:
MFCD00040474
EC Number:
1.1.1.8 (BRENDA, IUBMB)

Key Documents

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Product Name

α-Glycerophosphate Dehydrogenase from rabbit muscle, Type I, ammonium sulfate suspension, 100-300 units/mg protein

biological source

rabbit muscle

type

Type I

form

ammonium sulfate suspension

specific activity

100-300 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Protein determined by biuret.

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:
  • in the reaction mixture to measure the glycerol kinase activity
  • to demonstrate compartmentalized enzymatic reactions where NADH is involved
  • in the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling

Other Notes

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000512028
0000512028
0000489805
0000489805
0000446288

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Somchart Maenpuen et al.
The FEBS journal, 282(16), 3043-3059 (2015-02-26)
L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). The catalytic properties of recombinant His6-GlpO from Mycoplasma pneumoniae (His6-MpGlpO) were investigated through transient and
Joanne I Yeh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3280-3285 (2008-02-26)
Sn-glycerol-3-phosphate dehydrogenase (GlpD) is an essential membrane enzyme, functioning at the central junction of respiration, glycolysis, and phospholipid biosynthesis. Its critical role is indicated by the multitiered regulatory mechanisms that stringently controls its expression and function. Once expressed, GlpD activity
Structural studies on nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase crystallized from rat skeletal muscle.
T P Fondy et al.
The Journal of biological chemistry, 243(11), 3148-3160 (1968-06-10)
Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris
Aizemberg R, et al.
Food Technology and Biotechnology, 49(3), 329-329 (2011)
Laura L Grochowski et al.
Journal of bacteriology, 188(8), 2836-2844 (2006-04-06)
One of the early steps in the biosynthesis of coenzyme F(420) in Methanocaldococcus jannaschii requires generation of 2-phospho-L-lactate, which is formed by the phosphorylation of L-lactate. Preliminary studies had shown that L-lactate in M. jannaschii is not derived from pyruvate
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