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G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Brand: SIGMA

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym(s):

sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD⁺ 2-oxidoreductase

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About This Item

CAS Number:

9075-65-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-982-3

MDL number:

MFCD00040474

EC Number:

1.1.1.8 (BRENDA, IUBMB)

Specific activity:

100-300 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Level

200

type

Type I

form

ammonium sulfate suspension

specific activity

100-300 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

storage temp.

2-8°C

Description

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

in the reaction mixture to measure the glycerol kinase activity
to demonstrate compartmentalized enzymatic reactions where NADH is involved
in the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

Global Trade Item Number

SKU	GTIN
G6751-5KU	04061833640661
G6751-200UN	04061833640470
G6751-10KU	04061832730073
G6751-1KU	04061833640432