G6670
Anti-Growth Factor Independence-1 antibody, Mouse monoclonal
clone 2.5D.17, purified from hybridoma cell culture
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Synonym(s):
Anti-GFI1, Anti-ZNF163
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2.5D.17, monoclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
rat, mouse, human
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of A549 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)
Related Categories
General description
Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence
Specificity
The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).
Immunogen
rat growth factor independence-1 (GFI1).
Application
Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:
- immunoblotting
- immunohistochemistry
- EMSA (electrophoretic mobility shift analysis)
Biochem/physiol Actions
Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Analysis Note
The antibody may also be used in EMSA (electrophoretic mobility shift analysis).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22
Roberts T and Cowell JK
Oncogene, 14(8), 1003-1003 (1997)
Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
Doan LL, et al.
Nucleic Acids Research, 32(8), 2508-2519 (2004)
Loretta L Doan et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2356-2366 (2003-02-21)
T cell differentiation in the thymus is dependent upon signaling through the TCR and is characterized by the resulting changes in expression patterns of CD4 and CD8 surface coreceptor molecules. Although recent studies have characterized the effects of proximal TCR
Chinavenmeni S Velu et al.
Blood, 113(19), 4720-4728 (2009-03-13)
The zinc finger protein growth factor independent-1 (Gfi1) is a transcriptional repressor that is critically required for normal granulocytic differentiation. GFI1 loss-of-function mutations are found in some patients with severe congenital neutropenia (SCN). The SCN-associated GFI1-mutant proteins act as dominant
Loretta L Doan et al.
Nucleic acids research, 32(8), 2508-2519 (2004-05-08)
Growth factor independence-1 (GFI1) and GFI1B are closely related, yet differentially expressed transcriptional repressors with nearly identical DNA binding domains. GFI1 is upregulated in the earliest thymocyte precursors, while GFI1B expression is restricted to T lymphopoiesis stages coincident with activation.
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