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G6637

β-Galactose Dehydrogenase from Pseudomonas fluorescens

Name: β-Galactose Dehydrogenase from <I>Pseudomonas fluorescens</I>
Brand: SIGMA

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

Synonym(s):

D-Galactose:NAD⁺ 1-oxidoreductase

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About This Item

CAS Number:

9028-54-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-837-4

MDL number:

MFCD00130622

EC Number:

1.1.1.48 (BRENDA, IUBMB)

Specific activity:

≥50 units/mg protein (biuret)

Assay:

0.5—2.0 mg protein/mL (biuret)

Biological source:

Pseudomonas fluorescens

Recombinant:

expressed in E. coli

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Properties

biological source

Pseudomonas fluorescens

Quality Level

200

recombinant

expressed in E. coli

assay

0.5—2.0 mg protein/mL (biuret)

form

ammonium sulfate suspension

specific activity

≥50 units/mg protein (biuret)

color

white

suitability

suitable for enzyme test

application(s)

life science and biopharma

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Pseudomonas fluorescens ... gdh(533113295)

Description

Application

β-Galactose Dehydrogenase from Pseudomonas fluorescens has been used for competitive inhibition in lectin histochemistry. It has also been used to measure the hydrolysis activity of Haloferax alicantei β-galactosidase on different disaccharides.

Biochem/physiol Actions

β-galactose dehydrogenase catalyzes the oxidation of β-D-galactose to D-galactono-gammalactone.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 6.0

Other Notes

One unit will convert 1.0 μmole of D-galactose to D-galactonate per min at pH 8.6 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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以硫酸铵悬浮液形式提供的酶的使用指南

Affinity chromatography matures as bioinformatic and combinatorial tools develop.

Yannis D Clonis

Journal of chromatography. A, 1101(1-2), 1-24 (2005-10-26)

Affinity chromatography has the reputation of a more expensive and less robust than other types of liquid chromatography. Furthermore, the technique is considered to stand a modest chance of large-scale purification of proteinaceous pharmaceuticals. This perception is changing because of

Antisense suppression of l-galactose dehydrogenase in Arabidopsis thaliana provides evidence for its role in ascorbate synthesis and reveals light modulated l-galactose synthesis.

Stephan Gatzek et al.

The Plant journal : for cell and molecular biology, 30(5), 541-553 (2002-06-06)

l-Galactose dehydrogenase (l-GalDH), a novel enzyme that oxidizes l-Gal to l-galactono-1,4-lactone (l-GalL), has been purified from pea seedlings and cloned from Arabidopsis thaliana. l-GalL is a proposed substrate for ascorbate biosynthesis in plants, therefore the function of l-GalDH in ascorbate

Sensory properties and taste compounds of fermented milk produced by Lactococcus lactis and Streptococcus thermophius

M. Omaea et al.

Food Sci. Technol., 14, 183-189 (2008)

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Global Trade Item Number

SKU	GTIN
G6637-25UN	04061838255808