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Merck
CN

G5768

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Synonym(s):

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

NACRES:
NA.72
UNSPSC Code:
12352207

Key Documents

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Product Name

Gamborg′s B-5 Basal Salt Mixture, fine powder, suitable for plant cell culture

form

fine powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer
  • as a media to maintain potato suspension and callus cultures
  • as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

General description

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Preparation Note

Formulated to contain 3.1 grams of powder per liter of medium.
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

pictograms

Flame over circle
GHS03

signalword

Warning

hcodes

H272

Hazard Classifications

Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

危险化学品
动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000504749
0000504749
0000470198
0000470198
0000420644

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A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and
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Articles

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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