G5758
Glucose-6-Phosphatase from rabbit liver
≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)
Synonym(s):
D-Glucose-6-Phosphate Phosphohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
powder with sucrose
specific activity
≥0.05 units/mg protein
composition
Protein, ~30% (balance mostly sucrose)
storage temp.
−20°C
General description
Glucose-6-phosphatase is a multicomponent protein complex comprising of glucose-6-phosphate transporter (G6PT) and a catalytic unit (G6PC). Liver and kidney cells show predominant expression of glucose-6-phosphatase.
Application
Glucose-6-Phosphatase from rabbit liver has been used in glucose 6-phosphatase inhibitory assay of crude C. anisata leaf extracts and methanolic leaf and bark extracts of Dolichandrone atrovirens.
Glucose-6-Phosphatase was used to study the effects of Clausena anisata (Wild hook leaf) extracts on selected diabetes-related metabolizing enzymes.
Biochem/physiol Actions
Glucose-6-Phosphatase is crucial for regulating glucose homeostasis during starvation. Deficiency of glucose-6-phosphatase is implicated in glycogen storage disease type I (GSDI).
Glucose-6-phosphatase catalyzes the conversion of glucose-6-phosphate into glucose as the last step of gluconeogenesis.
Other Notes
It has been reported that glucose-6-phosphatase, inorganic pyrophosphatase, and pyrophosphate glucose phosphotransferase are associated with a single protein that catalyzes three different reactions:
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
Quality
Crude microsomal preparation
Unit Definition
One unit will release 1.0 μmole of inorganic phosphate from glucose 6-phosphate per min at pH 6.5 at 37 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Serrano Jr, AE
Aquaculture Nutrition, 19(4), 468-474 (2013)
Gonnie M Alkemade et al.
Diabetes, 62(3), 905-911 (2012-11-20)
It is generally believed that inflammatory cues can attract noncognate, "bystander" T-cell specificities to sites of inflammation. We have shown that recruitment of naive and in vitro activated autoreactive CD8⁺ T cells into endogenous islets requires local autoantigen expression. Here
EVIDENCE FOR THE COMMON IDENTITY OF GLUCOSE 6-PHOSPHATASE, INORGANIC PYROPHOSPHATASE, AND PYROPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE.
R C NORDLIE et al.
The Journal of biological chemistry, 239, 1680-1685 (1964-06-01)
Glucose-6-phosphatase catalytic subunit gene family
Hutton JC and O'Brien RM
The Journal of Biological Chemistry, 284(43), 29241-29245 (2009)
Emile van Schaftingen et al.
The Biochemical journal, 362(Pt 3), 513-532 (2002-03-07)
Glucose-6-phosphatase (G6Pase), an enzyme found mainly in the liver and the kidneys, plays the important role of providing glucose during starvation. Unlike most phosphatases acting on water-soluble compounds, it is a membrane-bound enzyme, being associated with the endoplasmic reticulum. In
Protocols
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
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