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Key Documents

Safety Information

G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Synonym(s):

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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5 UNITS
¥5,770.79
10 UNITS
¥9,952.52
25 UNITS
¥21,147.94

¥5,770.79


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5 UNITS
¥5,770.79
10 UNITS
¥9,952.52
25 UNITS
¥21,147.94

About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

¥5,770.79


Please contact Customer Service for Availability
New, lower price on this item!

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form

lyophilized powder

Quality Level

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

composition

Protein, 35-65% modified Warburg-Christian

impurities

≤10 % protein α-lactalbumin

storage temp.

−20°C

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1 of 4

This Item
L5385L2005L6010
specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

specific activity

-

specific activity

≥200 units/mg protein

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (essentially salt-free)

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

impurities

≤10 % protein α-lactalbumin

impurities

-

impurities

-

impurities

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

composition

Protein, 35-65% modified Warburg-Christian

composition

-

composition

-

composition

-

General description

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.[1]Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.[2]

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1[3]
  • in β -1,4-galactosyltransferase assay rice root cell extracts[4]
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers[5]

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides[6].
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality.[7] Alteration in the galactosyltransferase levels have been implicated in various disease states.[2]Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.[1]

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Unit Definition

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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The role of alpha-lactalbumin and the A protein in lactose synthetase: a unique mechanism for the control of a biological reaction.
K Brew et al.
Proceedings of the National Academy of Sciences of the United States of America, 59(2), 491-497 (1968-02-01)
Galactosyltransferase acceptor specificity of the lactose synthetase A protein.
F L Schanbacher et al.
The Journal of biological chemistry, 245(19), 5057-5061 (1970-10-10)
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
J A Campbell et al.
The Biochemical journal, 326 ( Pt 3), 929-939 (1997-10-23)
A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice
Jiang H, et al.
Plant Physiology, 138(1), 232-242 (2005)
The kinetic mechansim of bovine milk galactosyltransferase. The role of alpha-lactalbumin.
Bell JE, et al.
The Journal of Biological Chemistry, 251(10), 3003-3013 (1976)

Articles

Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.

Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.

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