G4798
Anti-GEP100 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-ARFGEP100, Anti-BRAG2, Anti-IQSEC1, IQ motif and Sec7 domain 1
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using whole extracts of human MDA-MB-231 and mouse 4T1 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... IQSEC1(9922)
mouse ... Iqsec1(232227)
General description
GEP100 is predominantly expressed in peripheral blood leukocytes, brain, and spleen. GEP100 contains an ARF-activating Sec7 domain, a pleckstrin homology (PH) domain and an Q-like motif.
Application
Anti-GEP100 antibody produced in rabbit is suitable for immunoblotting using human hepatoma cell line HepG2 to study the effect of GEP100/Arf6 on EGF-induced cell migration. It is suitable for western blot analysis at a working concentration of 1-2μg/mL using whole extracts of human MDA-MB-231 and mouse 4T1 cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
The protein interacts with a-catenin and regulates cell adhesion, actin cytoskeleton remodeling and cell migration. It also activates Arf6 during integrin internalization, which in turn regulates both endocytosis and recycling of β1 integrins.
Target description
GEP100 (also called BRAG2) is a guanine nucleotideexchangingfactor (GEF) for Arf6. GEP100 induces breast cancer invasion and metastasis by specifically activating Arf6 in response to EGF stimulation. GEP100 phosphorylates epidermal growth factor receptor (EGFR) by the direct binding of its PH domain to two phosphorylated tyrosines (Tyr 1068/1086) in EGFR, which in turn activates Arf6. Overexpression of GEP100 together with Arf6 causes non-invasive MCF7 cells to become highly invasive.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Jillian L Dunphy et al.
Current biology : CB, 16(3), 315-320 (2006-02-08)
The small GTPase Arf6 has been shown to regulate the post-endocytic trafficking of a subset of membrane proteins, including beta1 integrins, and inhibition of Arf6 function impairs both cell adhesion and motility. The activity of Arf GTPases is regulated by
Toyoko Hiroi et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(28), 10672-10677 (2006-06-30)
GEP(100) (p100) was identified as an ADP-ribosylation factor (ARF) guanine nucleotide-exchange protein (GEP) that partially colocalized with ARF6 in the cell periphery. p100 preferentially accelerated guanosine 5[gamma-thio]triphosphate (GTPgammaS) binding by ARF6, which participates in protein trafficking near the plasma membrane
Masaki Morishige et al.
Nature cell biology, 10(1), 85-92 (2007-12-18)
Epidermal growth factor (EGF) receptor (EGFR) signalling is implicated in tumour invasion and metastasis. However, whether there are EGFR signalling pathways specifically used for tumour invasion still remains elusive. Overexpression of Arf6 and its effector, AMAP1, correlates with and is
A Someya et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2413-2418 (2001-02-28)
A human cDNA encoding an 841-aa guanine nucleotide-exchange protein (GEP) for ADP-ribosylation factors (ARFs), named ARF-GEP(100), which contains a Sec7 domain, a pleckstrin homology (PH)-like domain, and an incomplete IQ-motif, was identified. On Northern blot analysis of human tissues, a
Jin Hwa Cho et al.
BMB reports, 46(12), 588-593 (2013-11-08)
Apoptosis, programmed cell death, is a process involved in the development and maintenance of cell homeostasis in multicellular organisms. It is typically accompanied by the activation of a class of cysteine proteases called caspases. Apoptotic caspases are classified into the
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