G4527
Gonadotropin from pregnant mare serum
lyophilized powder, BioXtra, suitable for mouse embryo cell culture
Synonym(s):
Equine gonadotropin, PMSG, Pregnant mare serum gonadotropin, eCG
sterility
aseptically processed
product line
BioXtra
form
lyophilized powder
potency
≥1000 I.U. per mg
specific activity
1,500-6,000 IU/mg
mol wt
~64 kDa
technique(s)
cell culture | embryo: suitable
solubility
saline: 1 mL/vial, H2O: soluble
shipped in
dry ice
storage temp.
−20°C
Application
Equine Gonadotropin, Pregnant Mares′ Serum (PMS-G) is used in conjunction with Human chorionic gonadotropin (hCG, Product No. C 8554) to superovulate female mice. PMS-G is used to promote egg follicle maturation by mimicking the effects of follicle stimulating hormone(FSH). For optimal egg production, hCG is injected 42 to 48 hours post PMS-G injection and is required for follicle rupture. Reconstitute 1 vial of PMS-G with 1 ml of 0.9% Sodium Chloride Solution (Product No. S 8776) to give a final concentration of 50 IU/ml. Intraperitoneally inject 0.1 ml into each animal. Product is qualified for use in mouse embryo preparation.
Biochem/physiol Actions
Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Stimulates the growth of ovarian follicles and the formation of the corpus luteum.
Two chain glycoprotein hormone; stimulates the growth of ovarian follicles and the formation of the corpus luteum.
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Regulatory Information
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Min-Hee Kang et al.
Data in brief, 28, 105025-105025 (2020-01-08)
To understand the effect of DMSO in preimplantation embryos, we have treated mouse 1 cell zygotes with DMSO and found that DMSO treatment caused 2 or 4 cell embryonic arrest and altered the acetylation levels of mouse preimplantation embryos To illustrate the
Kelly M Haston et al.
PloS one, 4(5), e5654-e5654 (2009-05-27)
Mammalian germ cells progress through a unique developmental program that encompasses proliferation and migration of the nascent primordial germ cell (PGC) population, reprogramming of nuclear DNA to reset imprinted gene expression, and differentiation of mature gametes. Little is known of
Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos.
Teraporn Vutyavanich et al.
Journal of assisted reproduction and genetics, 26(5), 285-290 (2009-07-16)
To compare closed-system solid surface vitrification with slow freezing. Mouse 2-cell embryos (n = 348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal
Hiwa Karimi et al.
Reproductive toxicology (Elmsford, N.Y.), 69, 196-203 (2017-03-13)
A study using a mouse IVF model was conducted to examine the hypothesis that in vitro fertilization (IVF) treatment may lead to immune system alteration in the offspring. Phagocytic activity and lymphocyte proliferative responses to mitogen, alloantigen, and purified protein
T Yamaguchi et al.
Jikken dobutsu. Experimental animals, 41(2), 153-159 (1992-04-01)
On the day of diestrus, female mice, syrian hamsters and rats, showing regular 4-day estrous cycles, were injected with hCG or PMSG and were inspected for the presence of ovulation the following day. The dose level expected to cause an
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