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G4388

Sigma-Aldrich

Glycerol 3-phosphate Oxidase from Streptococcus thermophilus

lyophilized powder, ≥35 units/mg solid, pH 7.0

Synonym(s):

sn-Glycerol 3-phosphate: oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

≥35 units/mg solid, pH 7.0

mol wt

131 kDa

shipped in

wet ice

storage temp.

−20°C

Application

GPO has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. GPO is part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.

Biochem/physiol Actions

Glycerol-3-phosphate oxidase catalyzes the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.

Unit Definition

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at pH 7.0 at 37 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form to Trypanosoma brucei.
F R Opperdoes et al.
European journal of biochemistry, 76(1), 29-39 (1977-06-01)
Shu-Yi Hsu et al.
Sensors (Basel, Switzerland), 10(6), 5758-5773 (2010-01-01)
A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized
Tatsuya Kishimoto et al.
Clinica chimica acta; international journal of clinical chemistry, 333(1), 59-67 (2003-06-18)
Several methods for measuring concentrations of lysophosphatidic acid (LPA), a lipid mediator, have been reported to date. However, these methods are not routinely used because most of them require specialized instrument and a complicated protocol. We developed a novel LPA
Alain Bizzini et al.
Journal of bacteriology, 192(3), 779-785 (2009-12-08)
Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone
C S Pundir et al.
Clinical biochemistry, 43(4-5), 467-472 (2009-12-29)
A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes. A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The

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