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G2N70

Sigma-Aldrich

GenElute™ Plant Genomic DNA Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonym(s):

Plant Genomic DNA Miniprep, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

usage

sufficient for 70 purifications

Quality Level

200

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

, Aligned

storage temp.

15-25°C

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General description

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots

GenElute™ Plant Genomic DNA Miniprep Kit has been used:
  • in the purification of genomic DNA isolated from neem tissues
  • to extract genomic DNA from embryos
  • to isolate DNA from plant samples

Features and Benefits

  • Starting material: Up to 100 mg of plant tissue
  • Expected yield: Up to 20 μg
  • Elution volume: 100 - 200 μl
  • Time required: < 40 min
  • RNase treatment required: No

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C

Supplementary Hazards

EUH032

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp.(Asteraceae)
SEViNDiK E, et al.
Notulae Scientia Biologicae , 8(4), 451-455 (2016)
The optimization of voltage parameter for tissue electroporation in somatic embryos of Astragalus chrysochlorus (Leguminosae)
Turgut-Kara N and Ari S
African Journal of Biotechnology, 9(29), 4584-4588 (2010)
Václav Bačovský et al.
International journal of molecular sciences, 23(5) (2022-03-11)
Young sex chromosomes possess unique and ongoing dynamics that allow us to understand processes that have an impact on their evolution and divergence. The genus Silene includes species with evolutionarily young sex chromosomes, and two species of section Melandrium, namely
Michael T Raissig et al.
The Plant cell, 23(1), 16-26 (2011-02-01)
Genomic imprinting results in monoallelic gene expression in a parent-of-origin-dependent manner. It is achieved by the differential epigenetic marking of parental alleles. Over the past decade, studies in the model systems Arabidopsis thaliana and maize (Zea mays) have shown a
Yang Zhu et al.
Nature communications, 11(1), 5118-5118 (2020-10-14)
Plants monitor seasonal cues to optimize reproductive success by tuning onset of reproduction and inflorescence architecture. TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) and their orthologs antagonistically regulate these life history traits, yet their mechanism of action, antagonism
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Protocols

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

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DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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