G1N9604
GenElute™ 96 Well Tissue Genomic DNA Purification Kit
4 x 96 well plate, sufficient for 4 96-well plate purifications
Synonym(s):
High Throughput Purification, Tissue Genomic DNA
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About This Item
Product Name
GenElute™ 96 Well Tissue Genomic DNA Purification Kit, 4 x 96 well plate, sufficient for 4 96-well plate purifications
usage
sufficient for 4 96-well plate purifications
quality
4 x 96 well plate
storage temp.
15-25°C
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Application
- PCR
- Southern blots
- cloning
- restriction digest
- enzymatic reactions
Biochem/physiol Actions
Features and Benefits
- Starting material: ≤ 20 mg tissue total (mouse, rat or whole sample) or ≤ 1 X 106 cultured cells or bacteria per well
- Average Expected yield: 10 - 20 μg/well (bacteria: 1.5 - 2.5 μg/well)
- Elution volume: 100 μl
- Time required: 70 min/plate
- Innovative wash plate minimizes risk of cross-contamination
- Processing possible under vacuum or centrifugation
- Suitable for manual and automated processing
General description
The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.
The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.
Legal Information
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
75.2 °F
flash_point_c
24 °C
Regulatory Information
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Protocols
This protocol is designed to allow for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues using the GenElute 96 Well Tissue Genomic DNA Purification Kit.
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