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G1910

Sigma-Aldrich

Gelzan CM

Gelrite®

Synonym(s):

Gellan Gum, Agar substitute gelling agent

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
10171502
NACRES:
NA.72

Quality Level

application(s)

agriculture

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General description

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.

Application

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological media. Gellan gum is used in a wide variety of immobilization matricies.
Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking
  • in the synthesis of gellan-pullulan hydrogel
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging

Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking
  • in the synthesis of gellan-pullulan hydrogel
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging

Features and Benefits

  • Transparent and colorless agar alternative
  • Mechanically robust
  • Disperses in water with ease
  • Low viscosity
  • The resulting gel has a high melting temperature

Legal Information

Gelrite is a registered trademark of CP Kelco U.S., Inc.
Gelzan is a trademark of CP Kelco U.S., Inc.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the difference between Product G1910, Gelzan CM and Gelrite?

    The products are chemically the same. However, Gelzan CM is produced through a new and more efficient process. There are some slight differences in the specifications of the two products.

  5. What is Product G1910, Gelzan CM?

    It is a low-acyl gellan gum used as a gelling agent in both microbiological assay media and tissue culture.

  6. Does Product G1910, Gelzan CM, have an expiration date?

    The lots of this product are not assigned expiration dates. The guarantee of quality is one year from the time of delivery for unopened bottles stored at room temperature.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. How do I use Product G1910, Gelzan CM?

    The G1910 is added to well-stirred water, and the dispersion heated to 85-90°C to ensure full hydration. To form a gel, the necessary amount of electrolyte, such as potassium citrate or calcium chloride, is added to the solution at 85°C and the mixture is poured or pumped into containers and allowed to cool undisturbed. The product is biodegradable, so a biocide is recommended. The typical use level is 0.2 to 2.0%.

  9. Is Product G1910, Gelzan CM, different that Gelrite?

    The G1910 is chemically the same as Gelrite, which is no longer produced. Both items are trademarked to CP Kelco U.S., Inc. They are both Gellan Gum, but the manufacturer has different specifications for these items.

  10. What is the gel strength of Product G1910, Gelzan CM?

    The gel strength as determined by the vendor is between 400-700 g/cm2.

  11. What is the particle size of Product G1910, Gelzan CM?

    The vendor supplied particle size is 42 mesh, or about 355 μm, with a specification of not less than 95% through. This means that at least 95% of the particles should be smaller than 355 microns in diameter.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Zhuoran Jiang et al.
ACS applied materials & interfaces, 11(31), 28289-28295 (2019-07-11)
Poly(tetrafluoroethylene) (PTFE) is a unique polymer with highly desirable properties such as resistance to chemical degradation, biocompatibility, hydrophobicity, antistiction, and low friction coefficient. However, due to its high melt viscosity, it is not possible to three-dimensional (3D)-print PTFE structures using
J T Oliveira et al.
Journal of tissue engineering and regenerative medicine, 3(7), 493-500 (2009-07-15)
Gellan gum is a polysaccharide that has been recently proposed by our group for cartilage tissue-engineering applications. It is commonly used in the food and pharmaceutical industry and has the ability to form stable gels without the use of harsh
João T Oliveira et al.
Tissue engineering. Part A, 16(1), 343-353 (2009-08-26)
Gellan gum is a polysaccharide that we have previously proposed for applications in the cartilage tissue engineering field. In this work, gellan gum hydrogels were tested for their ability to be used as injectable systems using simple processing methods, able
Timothy E L Douglas et al.
Journal of tissue engineering and regenerative medicine, 11(5), 1610-1618 (2015-07-16)
Gellan gum hydrogels functionalized with alkaline phosphatase were enzymatically mineralized with phosphates in mineralization medium containing calcium (Ca) and zinc (Zn) to improve their suitability as biomaterials for bone regeneration. The aims of the study were to endow mineralized hydrogels
Yi-Jun Qiao et al.
Plant diversity, 42(5), 323-333 (2020-11-03)
Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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