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G1767

Sigma-Aldrich

Glycogen from mussel, Mytilus genus

for DNA precipitations

Synonym(s):

DNA and RNA precipitation

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About This Item

CAS Number:
9005-79-2
EC Number:
232-683-8
MDL number:
MFCD00081547
UNSPSC Code:
12352200
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

liquid

packaging

vial of 1 mL

concentration

19-22 mg/mL

storage temp.

−20°C

InChI

1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1

InChI key

BYSGBSNPRWKUQH-UJDJLXLFSA-N

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General description

Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.

Application

Glycogen from Mytilus genus (Blue mussel) has been used


  • as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
  • as a reference standard in calibration curve generation to quantify hepatic glycogen content.
  • as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
  • for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
  • for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs

Biochem/physiol Actions

Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.

Features and Benefits

  • product provided is 1 mL total volume
  • precipitates even small amounts of DNA and RNA present in dilute solutions
  • does not interfere with OD 260/280 readings

Principle

Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

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Energy storage and reproduction in mussels, Mytilus galloprovincialis: the influence of diet quality
Fearman JA, et al.
Journal of shellfish research., 28(2), 305-312 (2009)
Regulation of gluconeogenesis by Kruppel-like factor 15
Gray S, et al.
Cell Metabolism, 5(4), 305-312 (2007)
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
Cruz C, et al.
eLife, 7, e34081-e34081 (2018)
Biomass composition of blue mussels, Mytilus edulis, is affected by living site and species of ingested microalgae
Pleissner D, et al.
ISRN zoology, 2012 (2012)
Tue Fryland et al.
Genome medicine, 8(1), 53-53 (2016-05-05)
The bromodomain containing 1 (BRD1) gene has been implicated with transcriptional regulation, brain development, and susceptibility to schizophrenia and bipolar disorder. To advance the understanding of BRD1 and its role in mental disorders, we characterized the protein and chromatin interactions
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