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G115080

Sigma-Aldrich

DMT-dG(dmf) Phosphoramidite

configured for PerkinElmer, configured for Polygen

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Synonym(s):
DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
Empirical Formula (Hill Notation):
C43H53N8O7P
CAS Number:
330628-04-1
Molecular Weight:
824.90
MDL number:
MFCD00797420
UNSPSC Code:
12352200
PubChem Substance ID:
329799827
NACRES:
NA.51

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

Assay

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)

form

powder

technique(s)

oligo synthesis: suitable

color

white to off-white

λ

conforms (UV/VIS Identity)

compatibility

configured for PerkinElmer
configured for Polygen

nucleoside profile

base: deoxyguanosine
base protecting group: DMF
2' protecting group: none
5' protecting group: DMT
deprotection: fast/standard

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6

InChI

1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1

InChI key

YRQAXTCBMPFGAN-UJASEYITSA-N

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General description

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamide (dmf) base protecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.Key Features of dG(dmf)
  • dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
  • The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
  • dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
  • dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
  • No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for Expedite™ and PolyGen® Synthesizers.

Legal Information

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
PolyGen is a registered trademark of PolyGen GmbH.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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