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Key Documents

Safety Information

G0154

Sigma-Aldrich

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

seawater medium

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250 ML
CN¥602.37

CN¥602.37

Available to ship onApril 27, 2025Details

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250 ML
CN¥602.37

About This Item

UNSPSC Code:
10171502
NACRES:
NA.72

CN¥602.37

Available to ship onApril 27, 2025Details

Request a Bulk Order

sterility

sterile-filtered

Quality Level

400

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

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489331999719999
Luperox® TBH70X, 叔丁基过氧化氢 溶液 70 wt. % in H2O

458139

Luperox® TBH70X, 叔丁基过氧化氢 溶液

叔丁基过氧化氢 溶液 ~5.5 M in decane

48933

叔丁基过氧化氢 溶液

叔丁基过氧化氢 溶液 packed in FEP bottles, ~5.5 M in decane (over molecular sieve 4Å)

19997

叔丁基过氧化氢 溶液

叔丁基过氧化氢 溶液 packed in FEP bottles, ~5.5 M in nonane (over molecular sieve 4 Å)

19999

叔丁基过氧化氢 溶液

concentration

12.25-13.31% (active oxygen), 70 wt. % in H2O, 69.0-75.0% (Na2S2O3, titration)

concentration

~5.5 M in decane

concentration

52.9-66.8% (iodometric), ~5.5 M in decane (over molecular sieve 4Å)

concentration

~5.5 M in nonane (over molecular sieve 4 Å)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

density

0.93 g/mL at 25 °C

density

-

density

0.83 g/mL at 20 °C

density

0.82 g/mL at 20 °C

form

liquid

form

-

form

liquid

form

liquid

refractive index

n20/D 1.386

refractive index

-

refractive index

n20/D 1.403

refractive index

n20/D 1.400

bp

96.2 °C

bp

-

bp

-

bp

-

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:
  • for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
  • to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
  • as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Formula variant

Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

Reconstitution

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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    Certificates of Analysis (COA)

    Lot/Batch Number
    RNBL7293
    RNBL0701
    RNBK0704
    RNBJ0050
    RNBH4653

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    Girish Beedessee et al.
    Scientific reports, 9(1), 1204-1204 (2019-02-06)
    Symbiodiniaceae dinoflagellates possess smaller nuclear genomes than other dinoflagellates and produce structurally specialized, biologically active, secondary metabolites. Till date, little is known about the evolution of secondary metabolism in dinoflagellates as comparative genomic approaches have been hampered by their large
    Gilles Defrel et al.
    Frontiers in bioengineering and biotechnology, 9, 734902-734902 (2021-10-19)
    Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host instability. Transgenic
    Willem Stock et al.
    FEMS microbiology ecology, 95(11) (2019-10-28)
    While different microalgae tend to be associated with different bacteria, it remains unclear whether such specific associations are beneficial for the microalgae. We assessed the impact of bacterial isolates, derived from various marine benthic diatoms, on the growth of several
    Shao-En Peng et al.
    PeerJ, 8, e9745-e9745 (2020-11-17)
    The mutualistic symbiosis between anthozoans and intra-gastrodermal dinoflagellates of the family Symbiodiniaceae is the functional basis of all coral reef ecosystems, with the latter providing up to 95% of their fixed photosynthate to their hosts in exchange for nutrients. However
    Amin R Mohamed et al.
    The ISME journal, 12(3), 776-790 (2018-01-13)
    Since the discovery of Chromera velia as a novel coral-associated microalga, this organism has attracted interest because of its unique evolutionary position between the photosynthetic dinoflagellates and the parasitic apicomplexans. The nature of the relationship between Chromera and its coral
    View All Related Papers

    Protocols

    Phycology Media in Plant Tissue Culture

    Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

    Antibiotics in Plant Tissue Culture Protocol

    Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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