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FLAAM

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

Synonym(s):

ATP assay kit, ATP detection kit

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

form

lyophilized powder

Quality Level

usage

 vial sufficient for ≥50 assays

technique(s)

PCR: suitable

storage temp.

−20°C

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Components

Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.

Reconstitution

Reconstitute with 5 ml of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anita Jemec et al.
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Within the FP7 EU project NanoValid a consortium of six partners jointly investigated the hazard of silver nanoparticles (AgNPs) paying special attention to methodical aspects that are important for providing high-quality ecotoxicity data. Laboratories were supplied with the same original
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The urothelium is a newly recognized sensory structure that detects bladder fullness. Pivotal to this sensory role is the release of ATP from the urothelium. However, the routes for urothelial ATP release, its modulation by receptor-mediated pathways, and the autocrine/paracrine
C J Williams et al.
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Buse Cevatemre et al.
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Despite the rapid advances in chemotherapy regimens, the outcome of patients with breast cancer is not satisfactory. One of the reasons of this dissatisfaction is that subsets of cells in tumors which referred as cancer stem cells (CSCs) show and/or
A trans-platinum (II) complex induces apoptosis in cancer stem cells of breast cancer
Aztopal N, et al.
Bioorganic & Medicinal Chemistry, 25(1), 269-276 (2017)

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