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FG0465

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IMDM

With stable-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

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Synonym(s):
Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium
UNSPSC Code:
12352207
NACRES:
NA.71

product name

Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

Quality Level

400

sterility

sterile-filtered
sterile

product line

AQmedia™

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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