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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
15121530
PubChem Substance ID:
NACRES:
NA.28
description
used in density gradient studies
Quality Level
density
1.93 g/mL at 25 °C (lit.)
SMILES string
FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F
InChI
1S/C15F33N/c16-1(17,4(22,23)10(34,35)36)7(28,29)13(43,44)49(14(45,46)8(30,31)2(18,19)5(24,25)11(37,38)39)15(47,48)9(32,33)3(20,21)6(26,27)12(40,41)42
InChI key
AQZYBQIAUSKCCS-UHFFFAOYSA-N
Application
Fluorinert™ FC-70 has been used to reduce the adherence of large trypsin:N-amidinopiperidine crystals to the well. It has also been used as a fluorocarbon oil for microinjection of oocytes.
For use as a high density chase solution in density gradient studies.
Packaging
Packaged and distributed by Sigma-Aldrich
Other Notes
Fluorinert FC-70 is a liquid mixture of completely fluorinated aliphatic compounds.
Legal Information
Fluorinert is a trademark of 3M Company
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Quantitative microinjection of oocytes, eggs, and embryos
Jaffe LA and Terasaki M
Methods in Cell Biology, 74, 219-242 (2004)
Alexandra Colin et al.
The Journal of cell biology, 219(3) (2020-01-18)
Nucleus centering in mouse oocytes results from a gradient of actin-positive vesicle activity and is essential for developmental success. Here, we analyze 3D model simulations to demonstrate how a gradient in the persistence of actin-positive vesicles can center objects of
Microinjection: Methods and Applications (2016)
Intriguing role of water in protein-ligand binding studied by neutron crystallography on trypsin complexes
Schiebel J, et al.
Nature Communications, 9 (2018)
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