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F9175

Flt-3/Flk-2 Ligand from mouse

≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.32
MDL number:
MFCD01093789

Key Documents

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Product Name

Flt-3/Flk-2 Ligand from mouse, ≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

biological source

mouse

recombinant

expressed in NSO cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

1.0-4.0 ng/mL

mol wt

~18 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P49772

storage temp.

−20°C

Quality Level

100

Gene Information

mouse ... Flt3l(14256)

Analysis Note

Bioactivity is measured by the ability to stimulate the proliferation of a Flt-3 transfected pro-B cell line.
Heterogenous glycosylation causes recombinant mouse Flt-3 ligand to migrate as multiple bands from 18-32 kDa.

Biochem/physiol Actions

Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myeloblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP and suppresses Erk signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.

General description

Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor. Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.
Mouse and human Flt-3 ligand share approximately 72% homology and both demonstrate cross-species activity.

Physical form

Lyophilized from a 0.2 μm filtered solution in 50% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
MKCL6580
MKBS0499V
MKBS1097V
MKBR8569V
110M1369

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S D Lyman et al.
Current opinion in hematology, 2(3), 177-181 (1995-05-01)
The flt3 ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cells. Other members of this family include Steel factor (also known as mast cell growth factor, stem cell factor, and kit
Yejing Ge et al.
Journal of cell science, 126(Pt 15), 3370-3379 (2013-05-25)
Various cues initiate multiple signaling pathways to regulate the highly coordinated process of skeletal myogenesis. Myoblast differentiation comprises a series of ordered events starting with cell cycle withdrawal and ending with myocyte fusion, with each step probably controlled by multiple
Yasuyuki Saito et al.
European journal of immunology, 43(6), 1651-1658 (2013-03-23)
Lymphoid-tissue dendritic cells (DCs) are short-lived and need to be continuously replenished from bone marrow-derived DC progenitor cells. Fms-related tyrosine kinase 3 is expressed during cellular development from hematopoietic progenitors to lymphoid-tissue DCs. Fms-related tyrosine kinase 3 ligand (Flt3L) is

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Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

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Data Sheet - F9175

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