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F8775

Sigma-Aldrich

Formaldehyde solution

for molecular biology, 36.5-38% in H2O

Synonym(s):

Formalin

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About This Item

Linear Formula:
HCHO
CAS Number:
50-00-0
Molecular Weight:
30.03
Beilstein:
1209228
EC Number:
200-001-8
MDL number:
MFCD00003274
UNSPSC Code:
12352114
PubChem Substance ID:
24894976
NACRES:
NA.31

grade

for molecular biology

Quality Level

100

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

form

liquid

autoignition temp.

572 °F

contains

10-15% methanol

concentration

36.5-38% in H2O

technique(s)

ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

density

1.09 g/mL at 25 °C (lit.)

suitability

suitable for (use in Formaldehyde-Agarose Gel Electrophoresis of RNA)

application(s)

general analytical
genomic analysis

storage temp.

15-25°C

SMILES string

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

Formaldehyde, a colorless, pungent-smelling chemical, finds extensive application in various biological research and laboratory settings. Primarily supplied as a 37% solution (formalin), its diverse uses range from tissue preservation and sterilization to acting as a denaturant in RNA gel electrophoresis. However, it′s crucial to acknowledge its potential health risks and handle it with appropriate precautions.Multifaceted Functions:
  • Preservative: Formaldehyde′s ability to inhibit microbial growth makes it an effective preservative in biological samples, preventing decay and ensuring their integrity for further analysis. This property finds application in medical laboratories, museums, and even embalming fluids.
  • Fixative: In histology, formaldehyde acts as a fixative, cross-linking proteins and preserving tissue structures for microscopic examination. This allows researchers to visualize anatomical details and cellular components with remarkable clarity.
  • Denaturant: In molecular biology techniques like formaldehyde-agarose gel electrophoresis, formaldehyde denatures RNA molecules, preventing them from forming secondary structures and enabling size-based separation. This technique proves invaluable for studying gene expression and RNA integrity.

Application

Formaldehyde solution has been used for fixing of cells and tissue sections. It has been used in the formaldehyde agarose gel electrophoresis. Formaldehyde is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Molecular Biology and Biochemical applications

Analysis Note

Tested for use in formaldehyde-agarose gel electrophoresis of RNA.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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comparable product

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Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Target Organs

Eyes,Central nervous system, Respiratory system

WGK

WGK 3

Flash Point(F)

133.0 °F - closed cup

Flash Point(C)

56.11 °C - closed cup

Regulatory Information

危险化学品

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